Part:BBa_K2951001:Design

Influenza B virus nucleoprotein

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1339
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 228
  Illegal SapI.rc site found at 776
  

Design Notes

1.Codon optimization: We optimized the sequence's codon usage for E.coli. 2.RFC 10 compatible: We checked for restriction sites for EcoRI, XbaI, SpeI, PstI and NotI in order to make it RFC compatible.

Source

The source of this part is from Influenza B virus (B/Colorado/16/2017) nucleoprotein (NP) gene, complete cds The sequence was obtained from NCBI